Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, hTERT-RPE1 cells, HeLa cells, human artery, mouse liver tissue, untreated RAW 264.7 cells, PMA and LPS-treated RAW 264.7 cells, mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4400 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83765-7-RR targets IL-33 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg7061 Product name: Recombinant human IL33 protein Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 122-270 aa of BC047085 Sequence: YLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 33 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 31 kDa, 18 kDa |
| GenBank accession number | BC047085 |
| Gene Symbol | IL-33 |
| Gene ID (NCBI) | 90865 |
| RRID | AB_3743495 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95760 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. IL-33 is synthesized as a 30 kDa full-length precursor that can be processed into an ~18-20 kDa mature form by inflammatory proteases, with the mature form exhibiting substantially higher biological activity as an extracellular alarmin cytokine.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-33 antibody 83765-7-RR | Download protocol |
| WB protocol for IL-33 antibody 83765-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |