Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | PMA, Ionomycin and Brefeldin A treated C57BL/6 Th2-polarized splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98655-2-RR targets IL-31 in FC (Intra) applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6811 Product name: Recombinant mouse IL-31 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-163 aa of NM_029594.1 Sequence: TCSLSFGAPISKEDLRTTIDLLKQESQDLYNNYSIKQASGMSADESIQLPCFSLDREALTNISVIIAHLEKVKVLSENTVDTSWVIRWLTNISCFNPLNLNISVPGNTDESYDCKVFVLTVLKQFSNCMAELQAKDNTTC 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 31 |
| Calculated molecular weight | 18 kDa |
| GenBank accession number | NM_029594.1 |
| Gene Symbol | Il31 |
| Gene ID (NCBI) | 76399 |
| RRID | AB_3746251 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6EAL8 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
IL-31 mRNA encodes a 164-aa precursor and a predicted 141-aa mature polypeptide, displaying an apparent molecular mass of 24 kDa in its glycosylated form. IL31 is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4+T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on. IL-31 signals through a heterodimeric receptor complex consisting of the IL31 receptor alpha (IL31Rα) and the oncostatin M receptor (OSMR). IL-31 plays a predominant function in the mediation of inflammatory and lymphoma-associated itch. Increased IL-31 expression has been detected in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IL-31 antibody 98655-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |