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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-28A Polyclonal antibody (22646-1-AP)

    WB analysis of Recombinant protein using 22646-1-AP (same clone as 22646-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 22646-1-AP (IL-28A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-28A Polyclonal antibody (22646-1-AP)

    IHC staining of human tonsillitis using 22646-1-AP (same clone as 22646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22646-1-AP (IL-28A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-28A Polyclonal antibody (22646-1-AP)

    IHC staining of human tonsillitis using 22646-1-AP (same clone as 22646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22646-1-AP (IL-28A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22646-1-PBS in a different storage buffer formulation.

IL-28A Polyclonal antibody, PBS Only

IL-28A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22646-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22646-1-PBS targets IL-28A in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18525

Product name: Recombinant human IL-28A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-91 aa of BC113583

Sequence: TVTGAVPVARLHGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCRCHSRLFPRTWDLRQLQV

相同性解析による交差性が予測される生物種
Full Name interleukin 28A (interferon, lambda 2)
Calculated molecular weight 200 aa, 22 kDa
GenBank accession numberBC113583
Gene Symbol IL-28A
Gene ID (NCBI) 282616
RRIDAB_2879141
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZJ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.