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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-2 Monoclonal antibody (60306-1-Ig)

    WB analysis using 60306-1-Ig (same clone as 60306-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IL-2 Monoclonal antibody (60306-1-Ig)

    WB analysis of Jurkat using 60306-1-Ig (same clone as 60306-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-2 Monoclonal antibody (60306-1-Ig)

    IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-2 Monoclonal antibody (60306-1-Ig)

    IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-2 Monoclonal antibody (60306-1-Ig)

    IHC staining of human breast cancer using 60306-1-Ig (same clone as 60306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-2 Monoclonal antibody (60306-1-Ig)

    IHC staining of human breast cancer using 60306-1-Ig (same clone as 60306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-2 Monoclonal antibody (60306-1-Ig)

    IF Staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using IL-2 Monoclonal antibody (60306-1-Ig)

    IF Staining of Jurkat using 60306-1-Ig (same clone as 60306-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

IL-2 Monoclonal antibody, PBS Only

IL-2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 60306-1-PBS
Clone No.2F11C5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

IL2, 2F11C5, IL 2, interleukin 2, Interleukin-2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60306-1-PBS targets IL-2 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10199

Product name: Recombinant human IL-2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-153 aa of BC070338

Sequence: MYRMQLLSCIALSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT

相同性解析による交差性が予測される生物種
Full Name interleukin 2
Calculated molecular weight 153 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC070338
Gene Symbol IL2
Gene ID (NCBI) 3558
RRIDAB_2881420
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP60568
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

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