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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using IL-1RN Recombinant antibody (83335-4-RR)

    WB analysis of mouse skin using 83335-4-RR

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 83335-4-RR (IL-1RN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using IL-1RN Recombinant antibody (83335-4-RR)

    IHC staining of mouse skin using 83335-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using IL-1RN Recombinant antibody (83335-4-RR)

    IHC staining of mouse skin using 83335-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83335-4-RR

    Affinity and Kinetic Characterization of 83335-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83335-4-RR against Mouse IL-1RN were performed. The affinity constant is 1.75 nM.

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IL-1RA  Recombinant antibody

IL-1RA  Recombinant Antibody for WB, IHC, ELISA
Cat No. 83335-4-RR
Clone No.240208G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

IL-1RN, Il1rn, Interleukin-1, IL-1RA, IL 1ra

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skin tissue
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83335-4-RR targets IL-1RA  in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 1 receptor antagonist
Calculated molecular weight18KD
Observed molecular weight18 kDa
GenBank accession numberNM_031167.5
Gene Symbol IL-1RA 
Gene ID (NCBI) 16181
RRIDAB_3670997
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ542W1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.

Protocols

Product Specific Protocols
WB protocol for IL-1RA  antibody 83335-4-RRDownload protocol
IHC protocol for IL-1RA  antibody 83335-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols