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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-17F Polyclonal antibody (30589-1-AP)

    WB analysis using 30589-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30589-1-AP (IL-17F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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IL-17F Polyclonal antibody

IL-17F Polyclonal Antibody for WB, ELISA
Cat No. 30589-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Il17f, interleukin 17F, IL 17F, C87042

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, EL-4 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30589-1-AP targets IL-17F in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 17F
Calculated molecular weight17KD
Observed molecular weight17-25 kDa
GenBank accession numberNM_145856.2
Gene Symbol IL-17F
Gene ID (NCBI) 257630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7TNI7-2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-17F, which is expressed by helper T cell 17(Th17) (at protein level). The expression pattern reflects the differentiation state. In fully differentiated Th17 cells, the level of IL-17a-IL-17f heterodimers is higher than that of IL-17a-IL-17a and IL-17f-IL-17f dimers (PMID:18025225), which are mainly secreted in the intestine (PMID:29915298). It is expressed by resident cells, epithelial cells and immune cell subsets of lamina propria (including natural killer cells, dendritic cells, macrophages and various T and B cell subsets) (PMID:16990136, PMID:29915298). The molecular weight of IL-17F is 17 kDa. IL-17F has the strongest similarity with IL-17A, and forms homodimer or heterodimer with IL-17A.

Protocols

Product Specific Protocols
WB protocol for IL-17F antibody 30589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols