Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84512-4-RR targets IL-15 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Product name: HumanKine® recombinant human IL-15 protein Source: -derived, Tag: Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 15 |
| Calculated molecular weight | 18 kDa |
| GenBank accession number | BC018149 |
| Gene Symbol | IL15 |
| Gene ID (NCBI) | 3600 |
| RRID | AB_3743576 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P40933 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-15 (IL-15) is a cytokine that plays a significant role in the immune system, particularly in the development, proliferation, and activation of immune cells such as natural killer (NK) cells, T cells, and B cells. It is a member of the immunoregulatory cytokines family and is predominantly expressed by myeloid cells, among other cell types. IL-15 serves multiple functions, including dictating T cell response, regulating tissue repair and B cell homing, modulating inflammation, and activating NK cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-15 antibody 84512-4-RR | Download protocol |
| WB protocol for IL-15 antibody 84512-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |


