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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-12B Polyclonal antibody (30956-1-AP)

    WB analysis using 30956-1-AP

    Human IL-12B/p40, IL-12/p70, IL-12A/p35 recombinant protein, were subjected to SDS PAGE followed by western blot with 30956-1-AP (IL-12B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP)

    WB analysis of HeLa using 30956-1-AP

    WB result of IL-12/IL-23 p40 antibody (30956-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12/IL-23 p40 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP)

    WB analysis using 30956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30956-1-AP (IL-12B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP)

    FC experiment of Jurkat using 30956-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of MOLT-4 cells using IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP)

    FC experiment of MOLT-4 using 30956-1-AP

    1x10^6 MOLT-4 cells were intracellularly stained with 0.4 ug IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

IL-12/IL-23 p40 Polyclonal antibody

IL-12/IL-23 p40 Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 30956-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

IL12B, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40, IL 12 subunit p40

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRecombinant protein, HepG2 cells, HeLa cells, Jurkat cells
Positive FC (Intra) detected inJurkat cells, MOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30956-1-AP targets IL-12/IL-23 p40 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg0431

Product name: Recombinant Human IL-12/IL-23 p40 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 23-328 aa of BC067498

Sequence: IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS

相同性解析による交差性が予測される生物種
Full Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Calculated molecular weight 328 aa, 37 kDa
Observed molecular weight40-45 kDa
GenBank accession numberBC067498
Gene Symbol IL12B
Gene ID (NCBI) 3593
RRIDAB_3669795
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP29460
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Protocols

Product Specific Protocols
FC protocol for IL-12/IL-23 p40 antibody 30956-1-APDownload protocol
WB protocol for IL-12/IL-23 p40 antibody 30956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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