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Validation Data Gallery

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  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Rat IL-12A/IL-12 p35 Rabbit Recombinant Antib (98594-2-RR)

    FC experiment of Transfected HEK-293T using 98594-2-RR

    1x10^6 HEK-293T cells (left) or IL-12A/IL-12 p35 Transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat IL-12A/IL-12 p35 Rabbit RecAb (98594-2-RR, Clone:250739E10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Flow cytometry (FC) experiment of C6 cells using Anti-Rat IL-12A/IL-12 p35 Rabbit Recombinant Antib (98594-2-RR)

    FC experiment of C6 using 98594-2-RR

    1x10^6 C6 cells were intracellularly stained with 0.25 ug Anti-Rat IL-12A/IL-12 p35 Rabbit RecAb (98594-2-RR, Clone: 250739E10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Rat IL-12A/IL-12 p35 Rabbit Recombinant Antib (98594-2-RR)

    FC experiment of Transfected HEK-293T using 98594-2-RR

    1x10^6 HEK-293T cells (left) or IL-12A/IL-12 p35 Transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat IL-12A/IL-12 p35 Rabbit RecAb (98594-2-RR, Clone: 250739E10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Affinity of 98594-2-RR

    Affinity of 98594-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98594-2-RR against Rat IL-12A/IL-12 p35 were performed. The affinity constant is below 1 pM.

Anti-Rat IL-12A/IL-12 p35 Rabbit Recombinant Antibody

IL-12A/IL-12 p35 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98594-2-RR
Clone No.250739E10

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Synonyms

CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, Il12a, IL-12A

Formulation:  PBS, Azide
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inC6 cells, Transfected HEK-293T cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98594-2-RR targets IL-12A/IL-12 p35 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3823

Product name: Recombinant Rat IL-12A protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 23-215 aa of NM_053390.1

Sequence: RVIPVSGPAKCLNQSQNLLKTTDDMVRTAREKLKHYSCTAGDIDHEDITRDKTSTLEACLPLELHKNESCLATKETSSIIRGSCLPPQKTSLMMTLCLGSIYEDLKMYQSEFQAINAALQSHNHQQITLDRNMLMAIDELMRSLNHSGETLHQKAPMGEADPYRVKMKLCILLHAFSTRVMTINRVMNYLSSS

相同性解析による交差性が予測される生物種
Full Name interleukin 12a
Calculated molecular weight24 kDa
GenBank accession numberNM_053390.1
Gene Symbol Il12a
Gene ID (NCBI) 84405
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9R103
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

Protocols

Product Specific Protocols
FC protocol for IL-12A/IL-12 p35 antibody 98594-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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