Validation Data Gallery
Tested Applications
| Positive WB detected in | Saos-2 supernatant cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68135-1-Ig targets IL-11 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Affinity | KD=1.80 x 10-9M KOff=2.20 x 10-6M KOn=1.22 x 103M |
| Immunogen |
CatNo: Ag11199 Product name: Recombinant human IL-11 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-199 aa of BC012506 Sequence: MNCVCRLVLVVLSLWPDTAVAPGPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAALAILGGLHLTLDWAVRGLLLLKTRL 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 11 |
| Calculated molecular weight | 199 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC012506 |
| Gene Symbol | IL-11 |
| Gene ID (NCBI) | 3589 |
| RRID | AB_2923662 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20809 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-11 (IL-11) is a member of the glycoprotein-130 (GP-130) cytokines that utilizes the GP-130 signaling pathway shared by other cytokines of the same family. The most well-known role of IL-11 is in hematopoiesis because it promotes megakaryocytopoiesis, erythropoiesis, and thrombopoiesis. In recognition of these properties, human IL-11 recombinant protein (rhIL-11) is used clinically for treating chemotherapy-induced thrombocytopenia. IL-11 has extensive immunomodulatory effects, both anti- and proinflammatory.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-11 antibody 68135-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
