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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using IKZF4 Recombinant monoclonal antibody (83471-5-RR)

    WB analysis of K-562 using 83471-5-RR (same clone as 83471-5-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IKZF4 Recombinant monoclonal antibody (83471-5-RR)

    WB analysis using 83471-5-RR (same clone as 83471-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse stomach tissue using IKZF4 Recombinant monoclonal antibody (83471-5-RR)

    IF Staining of mouse stomach using 83471-5-RR (same clone as 83471-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse stomach tissue using IKZF4 Recombinant monoclonal antibody (83471-5-RR)

    IF Staining of mouse stomach using 83471-5-RR (same clone as 83471-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using IKZF4 Recombinant monoclonal antibody (83471-5-RR)

    IF Staining of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using IKZF4 Recombinant monoclonal antibody (83471-5-RR)

    FC experiment of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug IKZF4 Recombinant antibody (83471-5-RR, Clone:240357D5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83471-5-RR

    Affinity and Kinetic Characterization of 83471-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83471-5-RR against Human IKZF4 were performed. The affinity constant is 21.3 nM.

IKZF4 Recombinant monoclonal antibody, PBS Only

IKZF4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 83471-5-PBS
Clone No.240357D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

240357D5, EOS, IKZF 4, KIAA1782, Zinc finger protein Eos

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83471-5-PBS targets IKZF4 in WB, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34767

Product name: Recombinant human IKZF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 268-457 aa of BC160169

Sequence: ERCHNYLQSLSTEAQALAGQPGDEIRDLEMVPDSMLHSSSERPTFIDRLANSLTKRKRSTPQKFVGEKQMRFSLSDLPYDVNSGGYEKDVELVAHHSLEPGFGSSLAFVGAEHLRPLRLPPTNCISELTPVISSVYTQMQPLPGRLELPGSREAGEGPEDLADGGPLLYRPRGPLTDPGASPSNGCQDST

相同性解析による交差性が予測される生物種
Full Name IKAROS family zinc finger 4 (Eos)
Observed molecular weight60 kDa
GenBank accession numberBC160169
Gene Symbol IKZF4
Gene ID (NCBI) 64375
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9H2S9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IKZF4 is a member of the Ikaros family transcription factors. The Ikaros family was originally designated to function in hematopoiesis, but it is only recently that studies have begun to show the relevance of this family in T helper cell differentiation. IKZF3 was preferentially expressed in TH17 cells and governs TH17 differentiation by silencing IL-2 expression. By investigating the dynamics of the TH17 regulatory network, IKZF4 was proposed by Yosef et al. as a composite in the negative regulatory module for TH17. (PMID: 24644282)

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