Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, SH-SY5Y cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
31833-1-AP targets IK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37095 Product name: Recombinant human IK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC013005 Sequence: MPERDSEPFSNPLAPDGHDVDDPHSFHQSKLTNEDFRKLLMTPRAAPTSAPPSKSRHHEMPREYNEDEDPAARRRKKKSYYAKLRQQEIERERELAEKYRDRAKERRDGVNKDYEETELISTTANYRAVGPTAEADKSAAEKRRQLIQESKFLGGDMEHTHLVKGLDFALLQKVRAEIASKEKEEEELMEKPQKETKKDEDPENKIEFKTRLGRNV 相同性解析による交差性が予測される生物種 |
| Full Name | IK cytokine, down-regulator of HLA II |
| Calculated molecular weight | 557 aa, 66 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC013005 |
| Gene Symbol | IK |
| Gene ID (NCBI) | 3550 |
| RRID | AB_3670123 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q13123 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IK, also known as the nuclear protein RED, is a down-regulator of human leukocyte antigen (HLA) II and interacts with the human SAC protein MAD1, which is required for the localization of the kinetochore of MAD1, for the progression of mitosis and for the activation of the SAC. IK is therefore an important protein for the correct progression of mitosis through the regulation of mitotic kinases and phosphatases. In addition, IK may be involved in pre-mRNA splicing, which may be a useful biomarker for new nuclear structures in cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IK antibody 31833-1-AP | Download protocol |
| IHC protocol for IK antibody 31833-1-AP | Download protocol |
| WB protocol for IK antibody 31833-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |