IHCeasy® TPRA1/GPR175 Ready-To-Use IHC Kit
TPRA1/GPR175 Ready-to-use reagent kit for IHC.
Reactivity
Human
Sample Type
FFPE tissue
Cat no : KHC3106
Synonyms
GPR175, Integral membrane protein GPR175, PP6566, TMEM227, TPRA1
Validation Data Gallery
Product Information
KHC3106 is a ready-to-use IHC kit for staining of TPRA1/GPR175. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy® TPRA1/GPR175 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
GPR175, also known as TPRA1 or TPRA40, is a transmembrane protein. GPR175 was first cloned as a GLP-1 receptor homolog in 3T3-L1 adipocytes and is also expressed in tissues that require Hh signaling during development, including the heart, brain, lung, pancreas, and muscle. Endogenous GPR175 localizes to cilia in a Hh-dependent manner, where it can interact with (constitutively) ciliary Gαi1 to inhibit the local production of cAMP by adenylyl cyclase.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | GPR175, Integral membrane protein GPR175, PP6566, TMEM227, TPRA1 |
