Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, U-118 MG cells, U-251 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18072-1-AP targets IGSF3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12711 Product name: Recombinant human IGSF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-352 aa of BC110651 Sequence: QVTVQEGPLYRTEGSHITIWCNVSGYQGPSEQNFQWSIYLPSSPEREVQIVSTMDSSFPYAIYTQRVRGGKIFIERVQGNSTLLHITDLQARDAGEYECHTPSTDKQYFGSYSAKMNLVVIPDSLQTTAMPQTLHRVEQDPLELTCEVASETIQHSHLSVAWLRQKVGEKPVEVISLSRDFMLHSSSEYAQRQSLGEVRLDKLGRTTFRLTIFHLQPSDQGEFYCEAAEWIQDPDGSWYAMTRKRSEGAVVNVQPTDKEFTVRLETEKRLHTVGEPVEFRCILEAQNVPDRYFAVSWAFNSSLIATMGPNAVPVLNSEFAHREARGQLKVA 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin superfamily, member 3 |
| Calculated molecular weight | 1195 aa, 135 kDa |
| Observed molecular weight | 135-140 kDa |
| GenBank accession number | BC110651 |
| Gene Symbol | IGSF3 |
| Gene ID (NCBI) | 3321 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75054 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGSF3, known as EWI3, is a member of the immunoglobulin superfamily (IgSF) involved in various cell-cell interactions. IGSF3 is part of a cell adhesion molecule that is involved in cell-to-cell recognition, binding, and signal transduction. IGSF3 plays an important role in neural development, especially in the migration of neural crest cells and the development of the enteric nervous system. (PMID: 9541533; PMID: 37821496).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGSF3 antibody 18072-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |