Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | human brain tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
14003-1-AP targets IGSF11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5094 Product name: Recombinant human IGSF11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 262-430 aa of BC034411 Sequence: GAFFYWRSKNKEEEEEEIPNEIREDDLPPKCSSAKAFHTEISSSDNNTLTSSNAYNSRYWSNNPKVHRNTESVSHFSDLGQSFSFHSGNANIPSIYANGTHLVPGQHKTLVVTANRGSSPQVMSRSNGSVSRKPRPPHTHSYTISHATLERIGAVPVMVPAQSRAGSLV 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin superfamily, member 11 |
| Calculated molecular weight | 430 aa, 46 kDa |
| Observed molecular weight | 40-50 kDa |
| GenBank accession number | BC034411 |
| Gene Symbol | IGSF11 |
| Gene ID (NCBI) | 152404 |
| RRID | AB_10603368 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5DX21 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGSF11 antibody 14003-1-AP | Download protocol |
| WB protocol for IGSF11 antibody 14003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurosci Lett Promotion of differentiation in developing mouse cerebellar granule cells by a cell adhesion molecule BT-IgSF.
| ||
Medicine (Baltimore) Prediction of lung squamous cell carcinoma immune microenvironment and immunotherapy efficiency with pyroptosis-derived genes |
