Validation Data Gallery

Filter: AllWBIHC

WB analysis of human plasma using 14678-1-AP
human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14678-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14678-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human spleen using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).
IHC staining of mouse spleen using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse spleen using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 14678-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

Ig Kappa Light Chain Polyclonal antibody

Ig Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14678-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA

Synonyms

IgG Kappa Light Chain, IGK, IGK-, IGKC, immunoglobulin kappa locus

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human plasma tissue, human liver tissue, mouse skeletal muscle tissue
Positive IHC detected in	human spleen tissue, human heart tissue, human placenta tissue, human skin tissue, human testis tissue, mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below
RIP	See 11 publications below

Product Information

14678-1-AP targets Ig Kappa Light Chain in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6389 Product name: Recombinant human IGK- protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC030813 Sequence: MEAPAQLLFLLLLWLPDTTGEIVMTQSPATLSVSPGERATLSCRASQSVTSNLAWYQQTPGQSPRLVIYGASSRASGVPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYNKWPHTFGQGTKLDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 相同性解析による交差性が予測される生物種
Full Name	immunoglobulin kappa locus
Calculated molecular weight	26 kDa
Observed molecular weight	26 kDa
GenBank accession number	BC030813
Gene Symbol	IgG Kappa Light Chain
Gene ID (NCBI)	50802
RRID	AB_1851235
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody detects the light chain (Kappa) of human immunoglobulin.

Protocols

Product Specific Protocols
IHC protocol for Ig Kappa Light Chain antibody 14678-1-AP	Download protocol
WB protocol for Ig Kappa Light Chain antibody 14678-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IF	Protein Cell Single-nucleus transcriptomics uncovers a geroprotective role of YAP in primate gingival aging Authors - Qinchao Hu View Article
	IHC	Nat Commun A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice. Authors - Christine Quach View Article
	IHC	PLoS Genet Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis. Authors - Hyun-Jin Na View Article
	RIP	J Bone Miner Metab Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes. Authors - Lanjuan Shen View Article
	RIP	Mol Med Rep Long non‑coding RNA XIST promotes cerebral ischemia/reperfusion injury by modulating miR‑27a‑3p/FOXO3 signaling. Authors - Hua Zhang View Article
	RIP	J Exp Clin Cancer Res RNA N6-methyladenosine reader IGF2BP2 promotes lymphatic metastasis and epithelial-mesenchymal transition of head and neck squamous carcinoma cells via stabilizing slug mRNA in an m6A-dependent manner. Authors - Dan Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human plasma using 14678-1-AP

WB analysis of human liver using 14678-1-AP

WB analysis of mouse skeletal muscle using 14678-1-AP

IHC staining of human spleen using 14678-1-AP

IHC staining of human spleen using 14678-1-AP

IHC staining of mouse spleen using 14678-1-AP

IHC staining of mouse spleen using 14678-1-AP

IHC staining of human heart using 14678-1-AP

IHC staining of human heart using 14678-1-AP

IHC staining of human placenta using 14678-1-AP

IHC staining of human placenta using 14678-1-AP

IHC staining of human testis using 14678-1-AP

IHC staining of human testis using 14678-1-AP

IHC staining of human skin using 14678-1-AP

IHC staining of human skin using 14678-1-AP

Ig Kappa Light Chain Polyclonal antibody

Ig Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Single-nucleus transcriptomics uncovers a geroprotective role of YAP in primate gingival aging

A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.

Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis.

Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes.

Long non‑coding RNA XIST promotes cerebral ischemia/reperfusion injury by modulating miR‑27a‑3p/FOXO3 signaling.

RNA N6-methyladenosine reader IGF2BP2 promotes lymphatic metastasis and epithelial-mesenchymal transition of head and neck squamous carcinoma cells via stabilizing slug mRNA in an m6A-dependent manner.