Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32157-1-AP targets IGFBP6 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg0087 Product name: Recombinant Human IGFBP6 protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 28-240 aa of BC011708 Sequence: RCPGCGQGVQAGCPGGCVEEEDGGSPAEGCAEAEGCLRREGQECGVYTPNCAPGLQCHPPKDDEAPLRALLLGRGRCLPARAPAVAEENPKESKPQAGTARPQDVNRRDQQRNPGTSTTPSQPNSAGVQDTEMGPCRRHLDSVLQQLQTEVYRGAQTLYVPNCDHRGFYRKRQCRSSQGQRRGPCWCVDRMGKSLPGSPDGNGSSSCPTGSSG 相同性解析による交差性が予測される生物種 |
| Full Name | insulin-like growth factor binding protein 6 |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC011708 |
| Gene Symbol | IGFBP6 |
| Gene ID (NCBI) | 3489 |
| RRID | AB_3670211 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P24592 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGFBP6, an O-glycosylated protein with a predicted molecular weight of 34 kDa, can inhibit cell proliferation through specific binding to IGF-II. IGFBP6 is a multifunctional protein that plays significant roles in various cellular activities, including immune response, tissue repair, and fibrosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGFBP6 antibody 32157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
