Various lysates were subjected to SDS PAGE followed by western blot with 83283-3-RR (IGFBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83283-3-PBS in a different storage buffer formulation.

Sandwich ELISA standard curve of MP00322-2, human IGFBP2 Recombinant Matched Antibody Pair - PBS only. 83283-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0823. 83283-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

Serum of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 109.5 ng/mL with a range of 47.4 - 199.1 ng/mL.

Urine of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 6251.0 pg/mL with a range of 306.2 - 19423.8 pg/mL.

MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 3750.9 pg/mL. MDA-MB-453 human breast carcinoma cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 9611.6 pg/mL.

Biolayer interferometry (BLl) kinetic assays of 83283-3-RR against Human IGFBP2 were performed. The affinity constant is 1.85 nM.