Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 20253-1-AP (same clone as 20253-1-PBS)
HepG2 cells lysates were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)
WB result of IGF2R-Specific antibody (20253-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IGF2R-Specific transfected HepG2 cells. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 20253-1-AP (same clone as 20253-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
WB analysis using 20253-1-AP (same clone as 20253-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)
IP result of anti-IGF2R-Specific (IP:20253-1-AP, 4ug; Detection:20253-1-AP 1:500) with HepG2 cells lysate 3480ug. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 20253-1-AP (same clone as 20253-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 20253-1-AP (same clone as 20253-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 20253-1-AP (same clone as 20253-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 20253-1-AP (same clone as 20253-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IGF2R-Specific antibody (20253-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IGF2R-Specific Polyclonal antibody (20253-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.
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1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IGF2R-Specific Polyclonal antibody (20253-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

IGF2R-Specific Polyclonal antibody, PBS Only

IGF2R-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 20253-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

IGF2R, CI MPR, CI Man-6-P receptor, CI Man 6 P receptor, CD222

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20253-1-PBS targets IGF2R-Specific in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	insulin-like growth factor 2 receptor
Calculated molecular weight	274 kDa
Observed molecular weight	300 kDa
GenBank accession number	NM_000876
Gene Symbol	IGF2R
Gene ID (NCBI)	3482
RRID	AB_10859779
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11717
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20253-1-AP (same clone as 20253-1-PBS)

WB analysis of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

WB analysis of L02 using 20253-1-AP (same clone as 20253-1-PBS)

WB analysis using 20253-1-AP (same clone as 20253-1-PBS)

IP experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

IHC staining of human liver cancer using 20253-1-AP (same clone as 20253-1-PBS)

IHC staining of human liver cancer using 20253-1-AP (same clone as 20253-1-PBS)

IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)

IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)

IHC staining of human liver using 20253-1-AP (same clone as 20253-1-PBS)

IHC staining of human liver using 20253-1-AP (same clone as 20253-1-PBS)

IF Staining of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

FC experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)