Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse retina using 11083-1-AP (same clone as 11083-1-PBS)
mouse retina tissue were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)
WB result of IFT57 antibody (11083-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFT57 transfected HEK-293 cells. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
WB analysis using 11083-1-AP (same clone as 11083-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)
IP result of anti-IFT57 (IP:11083-1-AP, 5ug; Detection:11083-1-AP 1:800) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.
IF Staining of hTERT-RPE1 using 11083-1-AP (same clone as 11083-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT57 antibody (11083-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

IFT57 Polyclonal antibody, PBS Only

IFT57 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11083-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Dermal papilla-derived protein 8, DERP8, ESRRBL1, Estrogen-related receptor beta-like protein 1, HIP1-interacting protein

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11083-1-PBS targets IFT57 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1566 Product name: Recombinant human IFT57 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-429 aa of BC011899 Sequence: MTAALAVVTTSGLEDGVPRSRGEGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPSRHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNATISNILSELRSFGRTADFPPSKLKSGYGEHVCYVLDCFAEEALKYIGFTWKRPIYPVEELEEESVAEDDAELTLNKVDEEFVEEETDNEENFIDLNVLKAQTYHLDMNETAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHRSGIESALKETKGFLDKLHNEITRTLEKISSREKYINNQLENLVQEYRAAQAQLSEAKERYQQGNGGVTERTRLLSEVMEELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVEMDIRIGIVEHTLLQSKLKEKSNMTRNMHATVIPEPATGFY 相同性解析による交差性が予測される生物種
Full Name	intraflagellar transport 57 homolog (Chlamydomonas)
Calculated molecular weight	49 kDa
Observed molecular weight	49 kDa
GenBank accession number	BC011899
Gene Symbol	IFT57
Gene ID (NCBI)	55081
RRID	AB_2121957
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NWB7
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse retina using 11083-1-AP (same clone as 11083-1-PBS)

WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

WB analysis using 11083-1-AP (same clone as 11083-1-PBS)

WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

IP experiment of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)

IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)

IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)

IHC staining of human pancreas using 11083-1-AP (same clone as 11083-1-PBS)

IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

IF Staining of hTERT-RPE1 using 11083-1-AP (same clone as 11083-1-PBS)

IFT57 Polyclonal antibody, PBS Only

IFT57 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™