Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 13615-1-AP
WB result of IFT20 antibody (13615-1-AP, 1:500) with si-Control and si-IFT20 transfected HEK293 cells.
WB analysis using 13615-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 13615-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13615-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MDCK using 13615-1-AP
MDCK cells were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 13615-1-AP
IP result of anti-IFT20 (IP:13615-1-AP, 3ug; Detection:13615-1-AP 1:700) with mouse testis tissue lysate 4000ug.
IHC staining of human endometrial cancer using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 13615-1-AP (IFT20 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human endometrial cancer using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 13615-1-AP (IFT20 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse brain using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat ovary using 13615-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of hTERT-RPE1 cells using 13615-1-AP
IF result (localization to Golgi-like structures) of anti-IFT20 (13615-1-AP, 1:50) with serum-starved hTERT-RPE1 (MoTH fixed) by Dr. Moshe Kim.
IF Staining of hTERT-RPE1 using 13615-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT20 antibody (13615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).
IF Staining of MDCK using 13615-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 13615-1-AP (IFT20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

IFT20 Polyclonal antibody

IFT20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13615-1-AP

Publications(90)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

hIFT20, Intraflagellar transport protein 20 homolog

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (90)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, MDCK cells, mouse testis tissue, rat testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	mouse brain tissue, rat ovary tissue, human placenta tissue, mouse ovary tissue, human ovary cancer tissue, human endometrial cancer tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	hTERT-RPE1 cells, MDCK cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 17 publications below
WB	See 52 publications below
IHC	See 7 publications below
IF	See 53 publications below
IP	See 3 publications below
CoIP	See 1 publications below

Product Information

13615-1-AP targets IFT20 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse, rat, canine, monkey, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4521 Product name: Recombinant human IFT20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-132 aa of BC038094 Sequence: MAKDILGEAGLHFDELNKLRVLDPEVTQQTIELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLKSIAKQREAQQQQLQALIAEKKMQLERYRVEYEALCKVEAEQNEFIDQFIFQK 相同性解析による交差性が予測される生物種
Full Name	intraflagellar transport 20 homolog (Chlamydomonas)
Calculated molecular weight	15 kDa
Observed molecular weight	15-18 kDa
GenBank accession number	BC038094
Gene Symbol	IFT20
Gene ID (NCBI)	90410
RRID	AB_2280001
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IY31
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.

Protocols

Product Specific Protocols
IF protocol for IFT20 antibody 13615-1-AP	Download protocol
IHC protocol for IFT20 antibody 13615-1-AP	Download protocol
IP protocol for IFT20 antibody 13615-1-AP	Download protocol
WB protocol for IFT20 antibody 13615-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature Functional interaction between autophagy and ciliogenesis. Authors - Pampliega Olatz O View Article
mouse	WB	Nat Aging A primary cilia-autophagy axis in hippocampal neurons is essential to maintain cognitive resilience Authors - Manon Rivagorda KD Validated View Article
mouse	IF	Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Authors - Quanlong Lu View Article
human	IF	Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Authors - Quanlong Lu View Article
human	WB	Nat Commun An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis Authors - Muqing Cao View Article
human	WB	Nat Commun PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. Authors - Asma Boukhalfa KD Validated View Article

Validation Data Gallery

WB analysis of HEK-293 using 13615-1-AP

WB analysis using 13615-1-AP

WB analysis using 13615-1-AP

WB analysis of HEK-293 using 13615-1-AP

WB analysis of MDCK using 13615-1-AP

IP experiment of mouse testis using 13615-1-AP

IHC staining of human endometrial cancer using 13615-1-AP

IHC staining of human endometrial cancer using 13615-1-AP

IHC staining of mouse brain using 13615-1-AP

IHC staining of mouse brain using 13615-1-AP

IHC staining of mouse brain using 13615-1-AP

IHC staining of mouse brain using 13615-1-AP

IHC staining of human ovary cancer using 13615-1-AP

IHC staining of human placenta using 13615-1-AP

IHC staining of mouse ovary using 13615-1-AP

IHC staining of rat brain using 13615-1-AP

IHC staining of rat ovary using 13615-1-AP

IF Staining of hTERT-RPE1 cells using 13615-1-AP

IF Staining of hTERT-RPE1 using 13615-1-AP

IF Staining of MDCK using 13615-1-AP

IFT20 Polyclonal antibody

IFT20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Functional interaction between autophagy and ciliogenesis.

A primary cilia-autophagy axis in hippocampal neurons is essential to maintain cognitive resilience

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis

PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress.

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