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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using IFT172 Recombinant antibody (86109-1-RR)

    WB analysis of mouse testis using 86109-1-RR (same clone as 86109-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 86109-1-RR (IFT172 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86109-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using IFT172 Recombinant antibody (86109-1-RR)

    WB analysis of mouse ovary using 86109-1-RR (same clone as 86109-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 86109-1-RR (IFT172 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86109-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT172 Recombinant antibody (86109-1-RR)

    IF Staining of hTERT-RPE1 using 86109-1-RR (same clone as 86109-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT172 antibody (86109-1-RR, Clone: 250711E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 86109-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86109-1-RR

    Affinity and Kinetic Characterization of 86109-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86109-1-RR against Human IFT172 were performed. The affinity constant is 2.02 nM.

IFT172 Recombinant monoclonal antibody, PBS Only

IFT172 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86109-1-PBS
Clone No.250711E6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

IFT, KIAA1179, NPHP17, osm 1, SLB

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86109-1-PBS targets IFT172 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag28921

Product name: Recombinant human IFT172 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1206-1408 aa of BC137126

Sequence: QARGALEEKDFQKAEGLLLRAQRPGLALNYYKEAGLWSDALRICKDYVPSQLEALQEEYEREATKKGARGVEGFVEQARHWEQAGEYSRAVDCYLKVRDSGNSGLAEKCWMKAAELSIKFLPPQRNMEVVLAVGPQLIGIGKHSAAAELYLNLDLVKEAIDAFIEGEEWNKAKRVAKELDPRYEDYVDQHYKEFLKNQGKVDS

相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 172 homolog (Chlamydomonas)
Calculated molecular weight 1749 aa, 198 kDa
Observed molecular weight180 kDa
GenBank accession numberBC137126
Gene Symbol IFT172
Gene ID (NCBI) 26160
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UG01
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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