IFT140 Recombinant antibody
IFT140 Recombinant Antibody for FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
FC, ELISA
Conjugate
Unconjugated
CloneNo.
230231A5
Cat no : 82947-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | U-251 cells, HepG2 cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
82947-2-RR targets IFT140 in FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | IFT140 fusion protein Ag11119 相同性解析による交差性が予測される生物種 |
Full Name | intraflagellar transport 140 homolog (Chlamydomonas) |
Calculated molecular weight | 1462 aa, 165 kDa |
GenBank accession number | BC035577 |
Gene symbol | IFT140 |
Gene ID (NCBI) | 9742 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)
Protocols
Product Specific Protocols | |
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FC protocol for IFT140 antibody 82947-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |