• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFT140 Recombinant antibody

IFT140 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230231F7

Cat no : 82947-1-RR

Synonyms

c305C8.4, c380F5.1, DKFZp564L232, FLJ10306, FLJ30571, gs114, IFT140, KIAA0590, WDTC2

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 82947-1-RR

    WB analysis of HeLa using 82947-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 82947-1-RR (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat testis using 82947-1-RR

    WB analysis of rat testis using 82947-1-RR

    rat testis tissue were subjected to SDS PAGE followed by western blot with 82947-1-RR (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

82947-1-RR targets IFT140 in WB, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFT140 fusion protein Ag11119 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight 1462 aa, 165 kDa
Observed molecular weight150-165 kDa
GenBank accession numberBC035577
Gene symbol IFT140
Gene ID (NCBI) 9742
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Protocols

Product Specific Protocols
WB protocol for IFT140 antibody 82947-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols