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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IFNAR1 Recombinant antibody (83002-4-RR)

    WB analysis of HeLa using 83002-4-RR (same clone as 83002-4-PBS)

    WB result of IFNAR1 antibody (83002-4-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFNAR1 transfected HeLa cells. This data was developed using the same antibody clone with 83002-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IFNAR1 Recombinant antibody (83002-4-RR)

    WB analysis using 83002-4-RR (same clone as 83002-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83002-4-RR (IFNAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83002-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using IFNAR1 Recombinant antibody (83002-4-RR)

    FC experiment of Jurkat using 83002-4-RR (same clone as 83002-4-PBS)

    1x10^6 Jurkat cells were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83002-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K562 using IFNAR1 Recombinant antibody (83002-4-RR)

    FC experiment of K562 using 83002-4-RR (same clone as 83002-4-PBS)

    1x10^6 K562 were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83002-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83002-4-RR

    Affinity and Kinetic Characterization of 83002-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83002-4-RR against Human IFNAR1 were performed. The affinity constant is 2.04 nM.

  • Featured Product
  • KD/KO Validated

IFNAR1 Recombinant monoclonal antibody, PBS Only

IFNAR1 Uni-rAb® Recombinant Antibody for WB, FC, Indirect ELISA
Cat No. 83002-4-PBS
Clone No.230549C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, Indirect ELISA

Synonyms

230549C8, AVP, CRF2 1, CRF2-1, Cytokine receptor class-II member 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83002-4-PBS targets IFNAR1 in WB, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFNAR1 fusion protein Eg0156 相同性解析による交差性が予測される生物種
Full Name interferon (alpha, beta and omega) receptor 1
Calculated molecular weight 557 aa, 64 kDa
Observed molecular weight80-140 kDa
GenBank accession numberBC021825
Gene Symbol IFNAR1
Gene ID (NCBI) 3454
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP17181
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.