Validation Data Gallery
Tested Applications
| Positive WB detected in | Transfected with IFNA8 HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87827-1-RR targets IFNA8 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg4954 Product name: Recombinant Human IFNA8 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 24-189 aa of BC104830 Sequence: CDLPQTHSLGNRRALILLAQMRRISPFSCLKDRHDFEFPQEEFDDKQFQKAQAISVLHEMIQQTFNLFSTKDSSAALDETLLDEFYIELDQQLNDLESCVMQEVGVIESPLMYEDSILAVRKYFQRITLYLTEKKYSSCAWEVVRAEIMRSFSLSINLQKRLKSKE 相同性解析による交差性が予測される生物種 |
| Full Name | interferon, alpha 8 |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC104830 |
| Gene Symbol | IFNA8 |
| Gene ID (NCBI) | 3445 |
| RRID | AB_3745773 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P32881 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFNA8 (Interferon alpha-8) is a member of the type I interferon family, primarily produced by macrophages and virus-infected cells, and possesses broad-spectrum antiviral activity. This protein exerts its effects by binding to the type I interferon receptor, activating the JAK-STAT signaling pathway, and inducing the expression of various interferon-stimulated genes (ISGs). Studies have shown that the antiviral activity of IFNA8 is significantly higher than that of other subtypes such as IFNA1, playing a crucial role in defending against infections like vesicular stomatitis virus. Furthermore, the transcription of IFNA8 is regulated by the transcription factor Oct-1, and single nucleotide polymorphisms in its promoter region are associated with the prognosis of glioma patients.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFNA8 antibody 87827-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |