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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IFNA14 Recombinant monoclonal antibody (87828-1-RR)

    WB analysis of Recombinant protein using 87828-1-RR (same clone as 87828-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 87828-1-RR (IFNA14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using IFNA14 Recombinant monoclonal antibody (87828-1-RR)

    WB analysis of HEK-293T using 87828-1-RR (same clone as 87828-1-PBS)

    Transfected with IFNA14 HEK-293T cells were subjected to SDS PAGE followed by western blot with 87828-1-RR (IFNA14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87828-1-PBS in a different storage buffer formulation.

  • Affinity of 87828-1-RR

    Affinity of 87828-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87828-1-RR against Human IFNA14 were performed. The affinity constant is 2.40 nM.

IFNA14 Recombinant monoclonal antibody, PBS Only

IFNA14 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87828-1-PBS
Clone No.252962C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IFN-alpha-14, Interferon alpha-14, Interferon alpha-H, Interferon lambda-2-H, LeIF H

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87828-1-PBS targets IFNA14 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg5295

Product name: recombinant human IFNA14 protein

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 24-189 aa of BC074956

Sequence: CNLSQTHSLNNRRTLMLMAQMRRISPFSCLKDRHDFEFPQEEFDGNQFQKAQAISVLHEMMQQTFNLFSTKNSSAAWDETLLEKFYIELFQQMNDLEACVIQEVGVEETPLMNEDSILAVKKYFQRITLYLMEKKYSPCAWEVVRAEIMRSLSFSTNLQKRLRRKD

相同性解析による交差性が予測される生物種
Full Name interferon, alpha 14
Observed molecular weight23 kDa
GenBank accession numberBC074956
Gene Symbol IFNA14
Gene ID (NCBI) 3448
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01570
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IFNA14 (Interferon α-14) is a type I interferon encoded by a gene located on chromosome 9 (9p21.3). It is mainly produced by macrophages and other cells. By binding to interferon receptors (IFNAR), it initiates signaling pathways such as JAK-STAT, playing a crucial role in antiviral immunity (especially against HIV-1), tumor occurrence, and immune regulation.

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