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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFITM2 Recombinant monoclonal antibody (83455-5-RR)

    WB analysis using 83455-5-RR (same clone as 83455-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83455-5-RR (IFITM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using IFITM2 Recombinant monoclonal antibody (83455-5-RR)

    WB analysis of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

    WB result of IFITM2 antibody (83455-5-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFITM2 transfected HepG2 cells. This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IFITM2 Recombinant monoclonal antibody (83455-5-RR)

    IF Staining of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IFITM2 Recombinant monoclonal antibody (83455-5-RR)

    IF Staining of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using IFITM2 Recombinant monoclonal antibody (83455-5-RR)

    FC experiment of MCF-7 using 83455-5-RR (same clone as 83455-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug IFITM2 Recombinant antibody (83455-5-RR, Clone:240318B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83455-5-RR

    Affinity and Kinetic Characterization of 83455-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83455-5-RR against Human IFITM2 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

IFITM2 Recombinant monoclonal antibody, PBS Only

IFITM2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83455-5-PBS
Clone No.240318B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

1 8D, 18D, 240318B6, DSPA2c, IFITM 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83455-5-PBS targets IFITM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3451

Product name: Recombinant human IFITM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-132 aa of BC009696

Sequence: MNHIVQTFSPVNSGQPPNYEMLKEEQEVAMLGAPHNPAPPTSTVIHIRSETSVPDHVVWSLFNTLFMNTCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGIFMTILLVIIPVLVVQAQR

相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 2 (1-8D)
Calculated molecular weight 132 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC009696
Gene Symbol IFITM2
Gene ID (NCBI) 10581
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ01629
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in a p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also, they serve as important components of the innate immune system to restrict HIV-1 infection.

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