Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, PC-3 cells, HeLa cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86924-1-RR targets IFIT5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4202 Product name: Recombinant human IFIT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 133-482 aa of BC025786 Sequence: YKLECPETDCEKGWALLKFGGKYYQKAKAAFEKALEVEPDNPEFNIGYAITVYRLDDSDREGSVKSFSLGPLRKAVTLNPDNSYIKVFLALKLQDVHAEAEGEKYIEEILDQISSQPYVLRYAAKFYRRKNSWNKALELLKKALEVTPTSSFLHHQMGLCYRAQMIQIKKATHNRPKGKDKLKVDELISSAIFHFKAAMERDSMFAFAYTDLANMYAEGGQYSNAEDIFRKALRLENITDDHKHQIHYHYGRFQEFHRKSENTAIHHYLEALKVKDRSPLRTKLTSALKKLSTKRLCHNALDVQSLSALGFVYKLEGEKRQAAEYYEKAQKIDPENAEFLTALCELRLSI 相同性解析による交差性が予測される生物種 |
| Full Name | interferon-induced protein with tetratricopeptide repeats 5 |
| Calculated molecular weight | 482 aa, 58 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC025786 |
| Gene Symbol | IFIT5 |
| Gene ID (NCBI) | 24138 |
| RRID | AB_3745208 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13325 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFIT5 antibody 86924-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |