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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IFIT1 Recombinant monoclonal antibody (83423-1-RR)

    WB analysis of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

    IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SGC-7901 cells using IFIT1 Recombinant monoclonal antibody (83423-1-RR)

    WB analysis of SGC-7901 using 83423-1-RR (same clone as 83423-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using IFIT1 Recombinant monoclonal antibody (83423-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR (same clone as 83423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using IFIT1 Recombinant monoclonal antibody (83423-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR (same clone as 83423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using IFIT1 Recombinant monoclonal antibody (83423-1-RR)

    FC experiment of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifit1 Recombinant Antibody (83423-1-RR, Clone:240132G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83423-1-RR

    Affinity and Kinetic Characterization of 83423-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83423-1-RR against Human IFIT1 were performed. The affinity constant is 0.397 nM.

IFIT1 Recombinant monoclonal antibody, PBS Only

IFIT1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83423-1-PBS
Clone No.240132G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

240132G11, G10P1, IFI 56K, IFI56, IFI-56K

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83423-1-PBS targets IFIT1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19691

Product name: Recombinant human IFIT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 340-460 aa of BC007091

Sequence: EVAHLDLARMYIEAGNHRKAEENFQKLLCMKPVVEETMQDIHFHYGRFQEFQKKSDVNAIIHYLKAIKIEQASLTRDKSINSLKKLVLRKLRRKALDLESLSLLGFVYKLEGNMNEALEYY

相同性解析による交差性が予測される生物種
Full Name interferon-induced protein with tetratricopeptide repeats 1
Calculated molecular weight 478 aa, 55 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC007091
Gene Symbol IFIT1
Gene ID (NCBI) 3434
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP09914
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

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