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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFI44 Recombinant antibody (83424-1-RR)

    WB analysis using 83424-1-RR (same clone as 83424-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83424-1-RR (IFI44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83424-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using IFI44 Recombinant antibody (83424-1-RR)

    FC experiment of A549 using 83424-1-RR (same clone as 83424-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug IFI44 Recombinant antibody (83424-1-RR, Clone:240022F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83424-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83424-1-RR

    Affinity and Kinetic Characterization of 83424-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83424-1-RR against Human IFI44 were performed. The affinity constant is 5.57 nM.

IFI44 Recombinant monoclonal antibody, PBS Only

IFI44 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83424-1-PBS
Clone No.240022F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

p44, MTAP44, Microtubule-associated protein 44, Interferon-induced protein 44, interferon induced protein 44

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83424-1-PBS targets IFI44 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26121

Product name: Recombinant human IFI44 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 9-175 aa of BC022870

Sequence: RLHEKILQNHFGGKRLSLLYKGSVHGFRNGVLLDRCCNQGPTLTVIYSEDHIIGAYAEESYQEGKYASIILFALQDTKISEWKLGLCTPETLFCCDVTKYNSPTNFQIDGRNRKVIMDLKTMENLGLAQNCTISIQDYEVFRCEDSLDERKIKGVIELRKSLLSALR

相同性解析による交差性が予測される生物種
Full Name interferon-induced protein 44
Calculated molecular weight 444 aa, 50 kDa
GenBank accession numberBC022870
Gene Symbol IFI44
Gene ID (NCBI) 10561
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ8TCB0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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