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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFI16 Monoclonal antibody

IFI16 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C10D7

Cat no : 67790-1-Ig

Synonyms

Ifi 16, IFI16, IFNGIP1, PYHIN2

Validation Data Gallery

Applications:
  • WB analysis using 67790-1-Ig

    WB analysis using 67790-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67790-1-Ig (IFI16 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67790-1-Ig

    WB analysis using 67790-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67790-1-Ig (IFI16 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67790-1-Ig

    IHC staining of human tonsillitis using 67790-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI16 antibody included
  • IHC staining of human tonsillitis using 67790-1-Ig

    IHC staining of human tonsillitis using 67790-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI16 antibody included
  • IHC staining of human tonsillitis using 67790-1-Ig

    IHC staining of human tonsillitis using 67790-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI16 antibody included
  • IHC staining of human renal cell carcinoma using 67790-1-Ig

    IHC staining of human renal cell carcinoma using 67790-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI16 antibody included
  • IHC staining of human renal cell carcinoma using 67790-1-Ig

    IHC staining of human renal cell carcinoma using 67790-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI16 antibody included
  • IF Staining of HeLa using 67790-1-Ig

    IF Staining of HeLa using 67790-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI16 antibody (67790-1-Ig, Clone: 1C10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MOLT-4 cells, Ramos cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IFI16 Ready-To-Use IHC Kit. IFI16 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67790-1-Ig targets IFI16 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFI16 fusion protein Ag30314 相同性解析による交差性が予測される生物種
Full Name interferon, gamma-inducible protein 16
Calculated molecular weight 729 aa, 82 kDa
Observed molecular weight85-95 kDa
GenBank accession numberBC017059
Gene symbol IFI16
Gene ID (NCBI) 3428
RRIDAB_2918554
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)

Protocols

Product Specific Protocols
WB protocol for IFI16 antibody 67790-1-IgDownload protocol
IHC protocol for IFI16 antibody 67790-1-IgDownload protocol
IF protocol for IFI16 antibody 67790-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols