Validation Data Gallery
Tested Applications
| Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
23849-1-AP targets IER2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20869 Product name: Recombinant human IER2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-223 aa of BC072432 Sequence: MEVQKEAQRIMTLSVWKMYHSRMQRGGLRLHRSLQLSLVMRSARELYLSAKVEALEPEVSLPAALPSDPRLHPPREAESTAETATPDGEHPFPEPMDTQEAPTAEETSACCAPRPAKVSRKRRSSSLSDGGDAGLVPSKKARLEEKEEEEGASSEVADRLQPPPAQAEGAFPNLARVLQRRFSGLLNCSPAAPPTAPPACEAKPACRPADSMLNVLVRAVVAF 相同性解析による交差性が予測される生物種 |
| Full Name | immediate early response 2 |
| Calculated molecular weight | 223 aa, 24 kDa |
| GenBank accession number | BC072432 |
| Gene Symbol | IER2 |
| Gene ID (NCBI) | 9592 |
| RRID | AB_2879339 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9BTL4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ier2 (also called ETR101, Pip92, 3CH92 and CHX1, hereafter referred to as Ier2, regardless of species) is an immediate early gene (PMID: 2678945) that was originally found to be upregulated in fibroblasts upon their activation (PMID: 3841511). Immediate early genes are a group of genes that are rapidly induced in quiescent cells by proliferation- and migration-inducing stimuli. Ier2 may have a role in tumor cell motility and metastasis (PMID: 22120713).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IER2 antibody 23849-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FEBS J PP2A-B55 and its adapter proteins IER2 and IER5 regulate the activity of RB family proteins and the expression of cell cycle-related genes | ||
Cell Signal IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A. |