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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDUA Polyclonal antibody (55158-1-AP)

    WB analysis using 55158-1-AP (same clone as 55158-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55158-1-AP (IDUA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using IDUA Polyclonal antibody (55158-1-AP)

    WB analysis of mouse brain using 55158-1-AP (same clone as 55158-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55158-1-AP (IDUA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IDUA Polyclonal antibody (55158-1-AP)

    IHC staining of human prostate cancer using 55158-1-AP (same clone as 55158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55158-1-AP (IDUA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IDUA Polyclonal antibody (55158-1-AP)

    IHC staining of human prostate cancer using 55158-1-AP (same clone as 55158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55158-1-AP (IDUA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55158-1-PBS in a different storage buffer formulation.

IDUA Polyclonal antibody, PBS Only

IDUA Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55158-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55158-1-PBS targets IDUA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name iduronidase, alpha-L-
Calculated molecular weight 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberNM_000203
Gene Symbol IDUA
Gene ID (NCBI) 3425
RRIDAB_11182946
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35475
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IDUA belongs to the glycosyl hydrolase 39 family. It hydrolysis of unsulfated alpha-L-iduronosidic linkages in dermatan sulfate. Defects in IDUA are the cause of mucopolysaccharidosis type 1H (MPS1H)( also known as Hurler syndrome), MPS1H/S(also known as Hurler-Scheie syndrome) and MPS1S(also known as Scheie syndrome), which are rare lysosomal storage diseases. This antibody is specific to IDUA.

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