Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
30530-1-AP targets IDO2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33013 Product name: Recombinant human IDO2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 268-420 aa of NM_194294 Sequence: GVSQEPLKYSGGSAAQSTVLHAFDEFLGIRHSKESGDFLYRMRDYMPPSHKAFIEDIHSAPSLRDYILSSGQDHLLTAYNQCVQALAELRSYHITMVTKYLITAAAKAKHGKPNHLPGPPQALKDRGTGGTAVMSFLKSVRDKTLESILHPRG 相同性解析による交差性が予測される生物種 |
| Full Name | indoleamine 2,3-dioxygenase 2 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 40-47 kDa |
| GenBank accession number | NM_194294 |
| Gene Symbol | IDO2 |
| Gene ID (NCBI) | 169355 |
| RRID | AB_3086352 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZQW0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IDO2 is one of two closely related tryptophan catabolizing enzymes induced under inflammatory conditions. In contrast to the immunoregulatory role defined for IDO1 in cancer models, IDO2 has a proinflammatory function in models of autoimmunity and contact hypersensitivity. The product of the IDO2 gene is a 420 amino-acid protein, with a molecular weight of ~47 kDa. (PMID: 34965962, PMID: 29807576, PMID: 35493480)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IDO2 antibody 30530-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |