×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse colon tissue using IDH3G Polyclonal antibody (25848-1-AP)

    WB analysis of mouse colon using 25848-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using IDH3G Polyclonal antibody (25848-1-AP)

    WB analysis of A431 using 25848-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using IDH3G Polyclonal antibody (25848-1-AP)

    WB analysis of A375 using 25848-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IDH3G Polyclonal antibody (25848-1-AP)

    IHC staining of human skin cancer using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IDH3G Polyclonal antibody (25848-1-AP)

    IHC staining of human skin cancer using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using IDH3G Polyclonal antibody (25848-1-AP)

    IHC staining of human colon using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using IDH3G Polyclonal antibody (25848-1-AP)

    IHC staining of human colon using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using IDH3G Polyclonal antibody (25848-1-AP)

    IHC staining of human colon using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 40x lens).

IDH3G Polyclonal antibody

IDH3G Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25848-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

NAD(+)-specific ICDH subunit gamma, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H IDHG

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, A375 cells, A431 cells
Positive IHC detected inhuman colon tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

25848-1-AP targets IDH3G in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23113

Product name: Recombinant human IDH3G protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-393 aa of BC000933

Sequence: MALKVATVAGSAAKAVLGPALLCRPWEVLGAHEVPSRNIFSEQTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIRVINGRAVEA

相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 3 (NAD+) gamma
Calculated molecular weight 43 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC000933
Gene Symbol IDH3G
Gene ID (NCBI) 3421
RRIDAB_2880267
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51553
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for IDH3G antibody 25848-1-APDownload protocol
WB protocol for IDH3G antibody 25848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep Med

Acutely denervated muscle EVs reshape neuronal mitochondrial metabolism via retrograde signaling to rescue peripheral nerve injury.

Authors - Qi Liu
View Article
humanWB

Oncogene

Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.

Authors - Ke Tang
View Article
||
New chat

Able™

正在加载，请稍候...