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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis using 23254-1-AP (same clone as 23254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of mouse brain using 23254-1-AP (same clone as 23254-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of DU 145 using 23254-1-AP (same clone as 23254-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of HEK-293 using 23254-1-AP (same clone as 23254-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of mouse liver using 23254-1-AP (same clone as 23254-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of Jurkat using 23254-1-AP (same clone as 23254-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using IDH2 Polyclonal antibody (23254-1-AP)

    IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using IDH2 Polyclonal antibody (23254-1-AP)

    IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IDH2 Polyclonal antibody (23254-1-AP)

    IF Staining of HepG2 using 23254-1-AP (same clone as 23254-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23254-1-AP (IDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

IDH2 Polyclonal antibody, PBS Only

IDH2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23254-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:1.1.1.42, ICD M, ICD-M, IDH 2, IDHM

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23254-1-PBS targets IDH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19748

Product name: Recombinant human IDH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 394-452 aa of BC009244

Sequence: FAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ

相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 41-47 kDa
GenBank accession numberBC009244
Gene Symbol IDH2
Gene ID (NCBI) 3418
RRIDAB_2879241
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48735
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 contains an N-terminal mitochondrial addressing sequence and hence is imported to the mitochondrial matrix, although localization to nuclei has also been reported. Moreover, IDH2, like ~20% of other mitochondrial enzymes, is acetylated at lysines, which inactivates the enzymatic activity.

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