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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ID4 Polyclonal antibody (21803-1-AP)

    WB analysis using 21803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21803-1-AP (ID4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ID4 Polyclonal antibody (21803-1-AP)

    IHC staining of mouse testis using 21803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ID4 Polyclonal antibody (21803-1-AP)

    IHC staining of mouse testis using 21803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using ID4 Polyclonal antibody (21803-1-AP)

    IHC staining of rat testis using 21803-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using ID4 Polyclonal antibody (21803-1-AP)

    IHC staining of rat testis using 21803-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ID4 Polyclonal antibody

ID4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21803-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

bHLHb27, ID4, IDB4, Inhibitor of DNA binding 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, MDA-MB-231 cells, mouse testis tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21803-1-AP targets ID4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ID4 fusion protein Ag16883 相同性解析による交差性が予測される生物種
Full Name inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
Calculated molecular weight 161 aa, 17 kDa
Observed molecular weight25-30 kDa
GenBank accession numberBC014941
Gene Symbol ID4
Gene ID (NCBI) 3400
RRIDAB_3085673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ID4 (inhibitor of DNA binding 4) negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. (PMID: 19783986). ID4 levels dictate the stem cell state in mouse spermatogonia (PMID: 28087628). It is involved in the regulation of many cellular processes during both prenatal development and tumorigenesis. ID4 can be detected 25-30 kDa (PMID: 34108007, PMID: 31847356).

Protocols

Product Specific Protocols
WB protocol for ID4 antibody 21803-1-APDownload protocol
IHC protocol for ID4 antibody 21803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols