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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ID3 Recombinant monoclonal antibody (86979-1-RR)

    WB analysis using 86979-1-RR (same clone as 86979-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86979-1-RR (ID3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86979-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02842-1

    Sandwich ELISA sample test of MP02842-1

    The mean ID3 concentration was determined to be 55.78 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 17.49 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02842-1

    Sandwich ELISA standard curve of MP02842-1

    Sandwich ELISA standard curve of MP02842-1, Human ID3 Recombinant Matched Antibody Pair - PBS only. 86979-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0588. 86979-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ID3 Recombinant monoclonal antibody (86979-1-RR)

    IF Staining of HeLa using 86979-1-RR (same clone as 86979-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ID3 antibody (86979-1-RR, Clone: 252047B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86979-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86979-1-RR

    Affinity and Kinetic Characterization of 86979-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86979-1-RR against Human ID3 were performed. The affinity constant is 0.184 nM.

ID3 Recombinant monoclonal antibody, PBS Only (Capture)

ID3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86979-1-PBS
Clone No.252047B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

1R21, bHLHb25, Class B basic helix-loop-helix protein 25, DNA-binding protein inhibitor ID-3, HEIR 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86979-1-PBS targets ID3 as part of a matched antibody pair:

MP02842-1: 86979-1-PBS capture and 86979-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0588

Product name: Recombinant human ID3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-119 aa of BC003107

Sequence: MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPRGTQLSQVEILQRVIDYILDLQVVLAEPAPGPPDGPHLPIQTAELAPELVISNDKRSFCH

相同性解析による交差性が予測される生物種
Full Name inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Calculated molecular weight119 aa, 13 kDa
Observed molecular weight 13-15 kDa
GenBank accession numberBC003107
Gene Symbol ID3
Gene ID (NCBI) 3399
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ02535
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The helix-loop-helix (HLH) family of transcription factors plays a central role in the regulation of cell growth, differentiation and tumourigenesis. Members of the Id (inhibitor of DNA binding) class of these nuclear proteins are able to heterodimerise with and thereby antagonise the functions of other transcription factors of this family. Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3, synonym: HEIR-1) is a member of the ID family of HLH proteins, which lacks a basic DNA-binding domain and inhibits transcription through formation of nonfunctional dimers that are incapable of binding to DNA.

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