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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ID1 Polyclonal antibody (18475-1-AP)

    WB analysis of PC-3 using 18475-1-AP (same clone as 18475-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using ID1 Polyclonal antibody (18475-1-AP)

    WB analysis of PC-3 using 18475-1-AP (same clone as 18475-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ID1 Polyclonal antibody (18475-1-AP)

    IHC staining of human placenta using 18475-1-AP (same clone as 18475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18475-1-AP (ID1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ID1 Polyclonal antibody (18475-1-AP)

    IHC staining of human placenta using 18475-1-AP (same clone as 18475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18475-1-AP (ID1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ID1 Polyclonal antibody (18475-1-AP)

    IF Staining of PC-3 using 18475-1-AP (same clone as 18475-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ID1 antibody (18475-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ID1 Polyclonal antibody, PBS Only

ID1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18475-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Inhibitor of DNA binding 1, Inhibitor of differentiation 1, ID, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18475-1-PBS targets ID1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ID1 fusion protein Ag13359 相同性解析による交差性が予測される生物種
Full Name inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Calculated molecular weight 16 kDa
Observed molecular weight20 kDa
GenBank accession numberBC000613
Gene Symbol ID1
Gene ID (NCBI) 3397
RRIDAB_2248812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41134
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 20 kDa.