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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICT1 Monoclonal antibody (68898-3-Ig)

    WB analysis using 68898-3-Ig (same clone as 68898-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ICT1 Monoclonal antibody (68898-3-Ig)

    WB analysis using 68898-3-Ig (same clone as 68898-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW480 cells using ICT1 Monoclonal antibody (68898-3-Ig)

    WB analysis of SW480 using 68898-3-Ig (same clone as 68898-3-PBS)

    SW480 cells were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using ICT1 Monoclonal antibody (68898-3-Ig)

    IF Staining of Caco-2 using 68898-3-Ig (same clone as 68898-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ICT1 antibody (68898-3-Ig, Clone: 1F11E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

ICT1 Monoclonal antibody, PBS Only

ICT1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68898-3-PBS
Clone No.1F11E6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

1F11E6, 39S ribosomal protein L58, mitochondrial, DS1, DS-1, EC:3.1.1.29

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68898-3-PBS targets ICT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33109

Product name: Recombinant human ICT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-206 aa of BC015335

Sequence: MAATRCLRWGLSRAGVWLLPPPARCPRRALHKQKDGTEFKSIYSLDKLYPESQGSDTAWRVPNGAKQADSDIPLDRLTISYCRSSGPGGQNVNKVNSKAEVRFHLATAEWIAEPVRQKIAITHKNKINRLGELILTSESSRYQFRNLADCLQKIRDMITEASQTPKEPTKEDVKLHRIRIENMNRERLRQKRIHSAVKTSRRVDMD

相同性解析による交差性が予測される生物種
Full Name immature colon carcinoma transcript 1
Calculated molecular weight 24 kDa
Observed molecular weight 21-24 kDa
GenBank accession numberBC015335
Gene Symbol ICT1
Gene ID (NCBI) 3396
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ14197
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells.

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