Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, rat lung tissue |
| Positive IP detected in | bEnd.3 cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31950-1-AP targets ICAM2/CD102 in WB, IHC, IP, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg1540 Product name: Recombinant Mouse ICAM-2/CD102 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 23-222 aa of NM_010494.2 Sequence: KAFEVYIWSEKQIVEATESWKINCSTNCAAPDMGGLETPTNKIMLEEHPQGKWKQFLVSNVSKDTVFFCHFTCSGKQHSESLNIRVYQPPAQVTLKLQPPRVFVGEDFTIECTVSPVQPLERLTLSLLRGRETLKNQTFGGAETVPQEATATFNSTALKKDGLNFSCQAELDLRPHGGYIIRSISEYQILEVYEPMQDNQ 相同性解析による交差性が予測される生物種 |
| Full Name | intercellular adhesion molecule 2 |
| Calculated molecular weight | 31kDa |
| Observed molecular weight | 45-55 kDa |
| GenBank accession number | NM_010494.2 |
| Gene Symbol | Icam2 |
| Gene ID (NCBI) | 15896 |
| RRID | AB_3670156 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P35330 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Intercellular adhesion molecule 2 (ICAM2) is also named CD102. ICAM2 is a type I transmembrane glycoprotein containing two immunoglobulin-like domains, which are crucial to lymphocyte recirculation and mediate cell survival signals via pathways associated with LFA-1 (PMID: 2497351, PMID: 11825565). ICAM2 has been reported to be a mediator for a survival signal sufficient to block apoptosis by activation of the PI3K/AKT pathway (PMID: 11825565). ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ICAM2/CD102 antibody 31950-1-AP | Download protocol |
| IP protocol for ICAM2/CD102 antibody 31950-1-AP | Download protocol |
| WB protocol for ICAM2/CD102 antibody 31950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |