Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, bEnd.3 cells |
| Positive IHC detected in | mouse cerebellum tissue, mouse lung tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84442-1-RR targets ICAM-2/CD102 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Fusion Protein 相同性解析による交差性が予測される生物種 |
| Full Name | intercellular adhesion molecule 2 |
| Calculated molecular weight | 31kDa |
| Observed molecular weight | 45-55 kDa |
| GenBank accession number | NM_010494.2 |
| Gene Symbol | Icam2 |
| Gene ID (NCBI) | 15896 |
| RRID | AB_3671967 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P35330 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ICAM-2/CD102 antibody 84442-1-RR | Download protocol |
| WB protocol for ICAM-2/CD102 antibody 84442-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |