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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICA1L Polyclonal antibody (18021-1-AP)

    WB analysis using 18021-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18021-1-AP (ICA1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using ICA1L Polyclonal antibody (18021-1-AP)

    WB analysis of mouse pancreas using 18021-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18021-1-AP (ICA1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ICA1L Polyclonal antibody (18021-1-AP)

    IHC staining of mouse brain using 18021-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18021-1-AP (ICA1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ICA1L Polyclonal antibody (18021-1-AP)

    IHC staining of mouse brain using 18021-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18021-1-AP (ICA1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ICA1L Polyclonal antibody

ICA1L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18021-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ALS2CR14, ALS2CR15, Islet cell autoantigen 1-like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse pancreas tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18021-1-AP targets ICA1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12570

Product name: Recombinant human ICA1L protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 133-482 aa of BC063876

Sequence: EVATFSQRAVSDTLMTINRMEQARTEYRGALLWMKDVSQELDPDTLKQMEKFRKVQMQVRNSKASFDKLKMDVCQKVDLLGASRCNMLSHSLTTYQRTLLGFWKKTARMMSQIHEACIGFHPYDFVALKQLQDTPSKISEDNKDEQIGGFLTEQLNKLVLSDEEASFESEQANKDHNEKHSQMREFGAPQFSNSENVAKDLPVDSLEGEDFEKEFSFLNNLLSSGSSSTSEFTQECQTAFGSPSASLTSQEPSMGSEPLAHSSRFLPSQLFDLGFHVAGAFNNWVSQEESELCLSHTDNQPVPSQSPKKLTRSPNNGNQDMSAWFNLFADLDPLSNPDAIGHSDDELLNA

相同性解析による交差性が予測される生物種
Full Name islet cell autoantigen 1,69kDa-like
Calculated molecular weight 482 aa, 54 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC063876
Gene Symbol ICA1L
Gene ID (NCBI) 130026
RRIDAB_2121543
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NDH6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ICA1L antibody 18021-1-APDownload protocol
IHC protocol for ICA1L antibody 18021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols