Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10797-1-AP targets ICA1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1219 Product name: Recombinant human ICA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-341 aa of BC005922 Sequence: MSGHKCSYPWDLQDRYAQDKSVVNKMQQKYWETKQAFIKATGKKEDEHVVASDADLDAKLELFHSIQRTCLDLSKAIVLYQKRICFLSQEENELGKFLRSQGFQDKTRAGKMMQATGKALCFSSQQRLALRNPLCRFHQEVETFRHRAISDTWLTVNRMEQCRTEYRGALLWMKDVSQELDPDLYKQMEKFRKVQTQVRLAKKNFDKLKMDVCQKVDLLGASRCNLLSHMLATYQTTLLHFWEKTSHTMAAIHESFKGYQPYEFTTLKVWKLEGPLENLEKSKVLFISQAKSIAESIIIPGLRMLKRELCPQQSLFLSFFLFFYCIRIQYNENLLQSKSAN 相同性解析による交差性が予測される生物種 |
| Full Name | islet cell autoantigen 1, 69kDa |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 60-65 kDa |
| GenBank accession number | BC005922 |
| Gene Symbol | ICA1 |
| Gene ID (NCBI) | 3382 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q05084 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Islet cell autoantigen 1 (ICA1), also known as ICA69, is a cytosolic and Golgi-bound protein. It controls neuroendocrine secretion and insulin granule trafficking via PICK1 interaction, and represents an autoantigen in type 1 diabetes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ICA1 antibody 10797-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |