Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells, Ramos cells |
Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84706-4-RR targets IBTK in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | IBTK fusion protein Ag35421 相同性解析による交差性が予測される生物種 |
Full Name | inhibitor of Bruton agammaglobulinemia tyrosine kinase |
Observed molecular weight | 160 kDa |
GenBank accession number | BC143745 |
Gene Symbol | IBTK |
Gene ID (NCBI) | 25998 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q9P2D0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IBTK, also known as BTKI and KIAA1417, which is involved in stress response and tumor growth. It has 3 isoforms and has been found to be hyper-expressed in chronic lymphocytic leukemia and correlated with disease progression (PMID: 29317636).
Protocols
Product Specific Protocols | |
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WB protocol for IBTK antibody 84706-4-RR | Download protocol |
IHC protocol for IBTK antibody 84706-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |