Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells, Ramos cells |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84706-4-RR targets IBTK in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag35421 Product name: Recombinant human IBTK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 850-1000 aa of BC143745 Sequence: VADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAKTKAKKKPRKRSDSSGGYNLSD 相同性解析による交差性が予測される生物種 |
| Full Name | inhibitor of Bruton agammaglobulinemia tyrosine kinase |
| Observed molecular weight | 160 kDa |
| GenBank accession number | BC143745 |
| Gene Symbol | IBTK |
| Gene ID (NCBI) | 25998 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9P2D0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IBTK, also known as BTKI and KIAA1417, which is involved in stress response and tumor growth. It has 3 isoforms and has been found to be hyper-expressed in chronic lymphocytic leukemia and correlated with disease progression (PMID: 29317636).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IBTK antibody 84706-4-RR | Download protocol |
| WB protocol for IBTK antibody 84706-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |