Validation Data Gallery

Filter: AllWBIHC-AutostainerIHCIF-PFC (Intra)

WB analysis using 81728-1-RR (same clone as 81728-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
WB analysis of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)
THP-1 cells were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of human tonsil using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsil slide using 81728-1-RR (IBA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of human tonsil using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR006). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR006). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)
1x10^6 THP-1 cells were intracellularly stained with 0.4 ug IBA1 Recombinant antibody (81728-1-RR, Clone:4D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
×
1x10^6 THP-1 cells were intracellularly stained with 0.4 ug IBA1 Recombinant antibody (81728-1-RR, Clone:4D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

IBA1 Recombinant monoclonal antibody, PBS Only

IBA1 Uni-rAb^® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF-P, FC (Intra), Indirect ELISA

Cat No. 81728-1-PBS

Clone No.4D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IHC-Autostainer, IF-P, FC (Intra), Indirect ELISA

Synonyms

AIF1, 4D5, AIF 1, AIF-1, allograft inflammatory factor 1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81728-1-PBS targets IBA1 in WB, IHC, IHC-Autostainer, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1363 Product name: Recombinant human IBA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC009474 Sequence: MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP 相同性解析による交差性が予測される生物種
Full Name	allograft inflammatory factor 1
Calculated molecular weight	17 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC009474
Gene Symbol	IBA1
Gene ID (NCBI)	199
ENSEMBL Gene ID	ENSG00000204472
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P55008
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 81728-1-RR (same clone as 81728-1-PBS)

WB analysis of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of human tonsil using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of human tonsil using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)

IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

IBA1 Recombinant monoclonal antibody, PBS Only

IBA1 Uni-rAb® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF-P, FC (Intra), Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

IBA1 Uni-rAb^® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF-P, FC (Intra), Indirect ELISA