Validation Data Gallery

Filter: AllIHC-AutostainerIHC

IHC staining of human brain using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human appendicitis using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human liver using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human lung using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human pituitary adenoma using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human pituitary adenoma using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human gliomas using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human gliomas using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human tonsillitis using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
IHC staining of human brain using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pituitary adenoma using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pituitary adenoma using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 66827-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

human IBA1 Monoclonal antibody

human IBA1 Monoclonal Antibody for IHC, ELISA

Cat No. 66827-1-Ig

Clone No.1C6A10

Publications(63)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

AIF1, IBA1, 1C6A10, AIF 1, AIF-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (63)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive IHC detected in

human brain tissue, human gliomas tissue, human liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IHC	See 7 publications below
IF	See 49 publications below

Product Information

66827-1-Ig targets human IBA1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28236 Product name: Recombinant human IBA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-147 aa of BC009474 Sequence: MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP 相同性解析による交差性が予測される生物種
Full Name	allograft inflammatory factor 1
Calculated molecular weight	17 kDa
GenBank accession number	BC009474
Gene Symbol	IBA1
Gene ID (NCBI)	199
ENSEMBL Gene ID	ENSG00000204472
RRID	AB_2882170
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P55008
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Protocols

Product Specific Protocols
IHC protocol for human IBA1 antibody 66827-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cancer Cell Distinct roles of TREM2 in central nervous system cancers and peripheral cancers Authors - Jian Zhong View Article
human	IHC	Nat Commun Fatal iatrogenic cerebral β-amyloid-related arteritis in a woman treated with lecanemab for Alzheimer's disease Authors - Elena Solopova View Article
	IF	Redox Biol CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-κB pathway Authors - Chengli Liu View Article
	IF	Theranostics An AIM2 inflammasome biomimetic mineralization inhibitor for vascular dementia therapy Authors - Yueqi Zhang View Article
	IF	Cell Death Dis ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis Authors - Yaxuan Zhang View Article
	IF	J Neuroinflammation Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice. Authors - Shiji Huo View Article

Validation Data Gallery

IHC staining of human brain using 66827-1-Ig

IHC staining of human appendicitis using 66827-1-Ig

IHC staining of human liver using 66827-1-Ig

IHC staining of human lung using 66827-1-Ig

IHC staining of human pituitary adenoma using 66827-1-Ig

IHC staining of human pituitary adenoma using 66827-1-Ig

IHC staining of human gliomas using 66827-1-Ig

IHC staining of human gliomas using 66827-1-Ig

IHC staining of human tonsillitis using 66827-1-Ig

IHC staining of human brain using 66827-1-Ig

IHC staining of human brain using 66827-1-Ig

IHC staining of human tonsillitis using 66827-1-Ig

IHC staining of human tonsillitis using 66827-1-Ig

IHC staining of human gliomas using 66827-1-Ig

IHC staining of human tonsillitis using 66827-1-Ig

IHC staining of human appendicitis using 66827-1-Ig

IHC staining of human liver using 66827-1-Ig

IHC staining of human brain using 66827-1-Ig

IHC staining of human lung using 66827-1-Ig

IHC staining of human gliomas using 66827-1-Ig

IHC staining of human pituitary adenoma using 66827-1-Ig

IHC staining of human pituitary adenoma using 66827-1-Ig

IHC staining of human liver cancer using 66827-1-Ig

IHC staining of human liver cancer using 66827-1-Ig

IHC staining of human gliomas using 66827-1-Ig

human IBA1 Monoclonal antibody

human IBA1 Monoclonal Antibody for IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Distinct roles of TREM2 in central nervous system cancers and peripheral cancers

Fatal iatrogenic cerebral β-amyloid-related arteritis in a woman treated with lecanemab for Alzheimer's disease

CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-κB pathway

An AIM2 inflammasome biomimetic mineralization inhibitor for vascular dementia therapy

ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis

Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.

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