Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, U2OS cells |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human testis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 2 publications below |
Product Information
26942-1-AP targets IARS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IARS fusion protein Ag25686 相同性解析による交差性が予測される生物種 |
| Full Name | isoleucyl-tRNA synthetase |
| Calculated molecular weight | 144 kDa |
| Observed molecular weight | 145 kDa |
| GenBank accession number | BC065552 |
| Gene Symbol | IARS |
| Gene ID (NCBI) | 3376 |
| RRID | AB_2880693 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41252 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IARS antibody 26942-1-AP | Download protocol |
| IHC protocol for IARS antibody 26942-1-AP | Download protocol |
| IP protocol for IARS antibody 26942-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation | ||
Food Chem Toxicol The crucial role of thioredoxin interacting protein in the liver insulin resistance induced by di (2-ethylhexyl) phthalates. | ||
Sci Rep Intermittent fasting activates macrophage migration inhibitory factor and alleviates high-fat diet-induced nonalcoholic fatty liver disease | ||
Discov Oncol High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer | ||
Nat Commun Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle | ||
Proc Natl Acad Sci U S A Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex
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