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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Huntingtin Monoclonal antibody (66553-1-Ig)

    IHC staining of human cerebellum using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Huntingtin Monoclonal antibody (66553-1-Ig)

    IHC staining of human cerebellum using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Huntingtin Monoclonal antibody (66553-1-Ig)

    IHC staining of human brain using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Huntingtin Monoclonal antibody (66553-1-Ig)

    IHC staining of human brain using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat cerebellum tissue using Huntingtin Monoclonal antibody (66553-1-Ig)

    IF Staining of rat cerebellum using 66553-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Huntingtin antibody (66553-1-Ig, Clone: 2F6C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), FUS/TLS antibody (11570-1-AP, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Huntingtin Monoclonal antibody (66553-1-Ig)

    IF Staining of SH-SY5Y using 66553-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Huntingtin antibody (66553-1-Ig, Clone: 2F6C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Huntingtin Monoclonal antibody

Huntingtin Monoclonal Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 66553-1-Ig
Clone No.2F6C9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IHC, IF/ICC, IF-P, ELISA

Synonyms

HTT, 2F6C9, HD, HD protein, Huntingtin, myristoylated N-terminal fragment

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat cerebellum tissue
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66553-1-Ig targets Huntingtin in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25922

Product name: Recombinant human Huntingtin protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 2845-3000 aa of NM_002111

Sequence: LDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCALRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKVSPGRTSDPNPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDIMNKVIGE

相同性解析による交差性が予測される生物種
Full Name huntingtin
Calculated molecular weight 348 kDa
GenBank accession numberNM_002111
Gene Symbol Huntingtin
Gene ID (NCBI) 3064
RRIDAB_2881915
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP42858
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for Huntingtin antibody 66553-1-IgDownload protocol
IHC protocol for Huntingtin antibody 66553-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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